Forestry Research Today is a free monthly online journal that collates and summarizes the latest research about Forestry, including details on fungi, diseases, management, harvesting. | ||||||
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Fungal radiation in the Cape Floristic Region: An analysis based on Gondwanamyces and Ophiostoma.Roets F, Wingfield MJ, Crous PW, Dreyer LL Department of Botany and Zoology, Stellenbosch University, Stellenbosch 7600, South Africa; Forestry and Agricultural Biotechnology Institute (FABI), Department of Microbiology, University of Pretoria, Pretoria 0002, South Africa. The Cape Floristic Region (CFR) displays high levels of plant diversity and endemism, and has received focused botanical systematic attention. In contrast, fungal diversity patterns and co-evolutionary processes in this region have barely been investigated. Here we reconstruct molecular phylogenies using the ITS and beta-tubulin gene regions of the ophiostomatoid fungi Gondwanamyces and Ophiostoma associated with southern African Protea species. Results indicate that they evolved in close association with Protea. In contrast to Protea, Ophiostoma species migrated to the CFR from tropical and subtropical Africa, where they underwent subsequent radiation. In both Gondwanamyces and Ophiostoma vector arthropods probably facilitated long-distance migration and shorter-distance dispersal. Although ecological parameters shaped most associations between ophiostomatoid fungi and Protea, there is congruence between fungal-host-associations and the systematic classification of Protea. These results confirm that the entire biotic environment must be considered in order to understand diversity and evolution in the CFR as a whole. Published 27 June 2008 in Mol Phylogenet Evol. Articles on Forestry published 26 June 2008: Assessment of large-scale deforestation of Nawarangpur district, Orissa, India: a remote sensing based study. Environ Monit Assess. The Nawarangpur district, Orissa, a trop ical region with Sal mixed moist deciduous and Sal mixed dry deciduous forests, has been affected by extensive deforestation. The district was surveyed using Landsat MSS (1973), Landsat TM (1990) and IRS P6 LISS III (2004) satellite imagery. From 1973 to 1990, more than 888.6 km(2) of dense forest (rate of deforestation = 3.62) and from 1990 to 2004, 429.7 km(2) (rate of deforestation = 3.97) were found to have been deforested. The analysis of results ... [Abstract] [Full-text] Articles on Forestry published 24 June 2008: Rapid and Efficient Gene Splicing Using Megaprimer-Based Protocol. Mol Biotechnol. Megaprimer-based methodology has been widely applied in site-directed mutagenesis, but rarely used in gene splicing. In this article, we describe a modification of the megaprimer PCR method, which can efficiently create and amplify a specific ligated chimeric gene segment in a PCR reaction and under a common PCR program that is widely used by researchers. More importantly, this modified method for splicing two or more gene fragments together revealed the mechanism of the megaprimer PCR method, ... [Abstract] [Full-text] Induced hydrolytic enzymes of ectomycorrhizal fungi against pathogen Rhizoctonia solani. Biotechnol Lett. Interactions between ectomycorrhizal fungi (Suillus laricinus, S. tomentosus, Amanita vaginata and Gomphidius viscidus) and the pathogen Rhizoctonia solani in co-culture were studied using both light and scanning electron microscopy. S. laricinus, S. tomentosus and A. vaginata inhibited the growth of the pathogen. Moreover, A. vaginata exhibited coiling around and penetration of the hyphae into R. solani was observed in the interaction zone. Furthermore, the production of chitinases, ... [Abstract] [Full-text] Indicators of carbon storage in u.s. Ecosystems: baseline for terrestrial carbon accounting. J Environ Qual, 37(4): 1376-82. Policymakers, program managers, and landowners need information about net terrestrial carbon sequestration in forests, croplands, grasslands, and shrublands to understand the cumulative effects of carbon trading programs, expanding biofuels production, and changing environmental conditions in addition to agricultural and forestry uses. Objective information systems that establish credible baselines and track changes in carbon storage can provide the accountability needed for carbon trading ... [Abstract] [Full-text] Avoided deforestation as a greenhouse gas mitigation tool: economic issues. J Environ Qual, 37(4): 1368-75. Tropical deforestation is a significant contributor to accumulation of greenhouse gases (GHGs) in the atmosphere. GHG emissions from deforestation in the tropics were in the range of 1 to 2 Pg C yr(-1) for the 1990s, which is equivalent to as much as 25% of global anthropogenic GHG emissions. While there is growing interest in providing incentives to avoid deforestation and consequently reduce net carbon emissions, there is limited information available on the potential costs of these ... [Abstract] [Full-text] Articles on Forestry published 23 June 2008: 3G vector-primer plasmid for constructing full-length-enriched cDNA libraries. Anal Biochem. We designed a 3G vector-primer plasmid for the generation of full-length-enriched complementary DNA (cDNA) libraries. By employing the terminal transferase activity of reverse transcriptase and the modified strand replacement method, this plasmid (assembled with a polydT end and a deoxyguanosine [dG] end) combines priming full-length cDNA strand synthesis and directional cDNA cloning. As a result, the number of steps involved in cDNA library preparation is decreased while simplifying downstream ... [Abstract] [Full-text] An N-terminal fragment of mouse DGAT1 binds different acyl-CoAs with varying affinity. Biochem Biophys Res Commun. A histidine-tagged recombinant N-terminal fragment of type-1 mouse liver diacylglycerol acyltransferase (DGAT; EC 2.3.1.20), MmDGAT1((1-95))His6, was expressed in Escherichia coli, and used to investigate possible acyl-CoA-binding properties. Analysis of the purified fragment by MALDI-TOF mass spectrometry revealed a polypeptide with molecular mass of about 11kDa which was consistent with the calculated molecular mass based on the deduced amino acid sequence. Lipidex-1000 binding assays ... [Abstract] [Full-text] Molecular cloning, identification, and chromosomal localization of two MADS box genes in peach (Prunus persica). J Genet Genomics, 35(6): 365-72. MADS box proteins play an important role in floral development. To find genes involved in the floral transition of Prunus species, cDNAs for two MADS box genes, PpMADS1 and PpMADS10, were cloned using degenerate primers and 5'- and 3'-RACE based on the sequence database of P. persica and P. dulcis. The full length of PpMADS1 cDNA is 1,071 bp containing an open reading frame (ORF) of 717 bp and coding for a polypeptide of 238 amino acid residues. The full length of PpMADS10 cDNA is 937 bp ... [Abstract] [Full-text] © 2005-2008 Forestry Research Today. All Rights Reserved. |
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