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Capillary zone electrophoresis method for fingerprint of allantoic fluid in normal and infected SPF embryonated chicken eggs.

Guan Y, Wang W, Zu Y, Fu Y, Liu J

Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Hexing Road 26, 150040 Harbin, China.

A capillary zone electrophoresis method was developed for fingerprint determination of allantoic fluid in specific pathogen free (SPF) embryonated chicken eggs. The effects of some crucial parameters, such as buffer type, pH, wavelength and running voltage on the separation were studied systematically. The components of the allantoic fluid were well separated using a fused-silica uncoated capillary with an effective length of 50cm and an internal diameter of 50mum. One hundred millimolars sodium tetraborate buffer containing 20mM sodium dihydrogen phosphate with a final pH 9.8 was used as a running buffer. Comparative fingerprints of allantoic fluid in normal and infected with infectious bronchitis virus (IBV) SPF embryonated chicken eggs were also evaluated. The results showed that there were significant differences between composition of normal allantoic fluid and allantoic fluid infected with IBV, which led to different migration behavior. This method was shown to be stable and reproducible with a relative standard deviation of less than 5% for both migration time and peak current.

Published 23 October 2006 in J Virol Methods.
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